The microbiome-macrophage interaction in health and disease
The aim of this sub-project is to understand the interaction of the microbiome with macrophages in human and murine health and disease. The group will contribute to the overarching goal of the SFB by providing crucial microbiome information to the consortium for their specific sub-projects (T. Weichhart, G. Egger, G. Schabbauer, M. Schweiger). Using human clinical samples (UC and CRC, G. Egger) and samples from mouse models (T. Weichhart, A. Haschemi), we will analyze the correlation of the bacterial, archaeal and fungal microbiome profile/function with macrophage functionality (E. Pohl) in disease and health. Specifically, we are exploring non-bacterial microbiome components (particularly archaea and fungi), and will assess, in a coordinated approach between T. Weichhart and E. Pohl, if and how archaeal and fungal cellular components and products can alter macrophage functionality in exposure assays. This information will finally feed into functional experiments, which aim for the identification of (archaeal) components or products, which can be used to reduce disease-patterns phenotypically in mouse models (T. Weichhart) or organoid models (G. Egger) and thus may constitute novel immunometabolic targets in human disease. By focusing our efforts on the non-bacterial microbiome, we aim to identify novel factors of macrophage interaction and novel possibilities to manipulate macrophage functionality through microbiome modulation.
Christine Moissl-Eichinger
Medical University of Graz (MUG)
Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine
Neue Stiftingtalstrasse 6
8010 Graz
christine.moissl-eichinger@medunigraz.at
https://www.moissleichingerlab.com/
https://www.medunigraz.at/en/team-christine-moissl-eichinger
Project Members
Torben Wilhelm Kühnast
Postdoc
SFB member
Viktoria Weinberger
PhD Student
SFB member
Research Publications
Exploring the human archaeome: its relevance for health and disease, and its complex interplay with the human immune system. Kuehnast, T; Kumpitsch, C; Mohammadzadeh, R; Weichhart, T; Moissl-Eichinger, C; Heine, H: FEBS J. 2024; https://doi.org/10.1111/febs.17123
Interindividual differences in aronia juice tolerability linked to gut microbiome and metabolome changes-secondary analysis of a randomized placebo-controlled parallel intervention trial. Lackner, S; Mahnert, A; Moissl-Eichinger, C; Madl, T; Habisch, H; Meier-Allard, N; Kumpitsch, C; Lahousen, T; Kohlhammer-Dohr, A; Mörkl, S; Strobl, H; Holasek, S. Microbiome. 2024; 12(1): 49; https://doi.org/10.1186/s40168-024-01774-4
Metabolic support by macrophages sustains colonic epithelial homeostasis. Fritsch SD, Sukhbaatar N, Gonzales K, Sahu A, Tran L, Vogel A, Mazic M, Wilson JL, Forisch S, Mayr H, Oberle R, Weiszmann J, Brenner M, Vanhoutte R, Hofmann M, Pirnes-Karhu S, Magnes C, Kühnast T, Weckwerth W, Bock C, Klavins K, Hengstschläger M, Moissl-Eichinger C, Schabbauer G, Egger G, Pirinen E, Verhelst SHL, Weichhart T. Cell Metab. 2023 Nov 7;35(11):1931-1943.e8. https://doi.org/10.1016/j.cmet.2023.09.010.
Clinical NEC prevention practices drive different microbiome profiles and functional responses in the preterm intestine. Neumann, CJ; Mahnert, A; Kumpitsch, C; Kiu, R; Dalby, MJ; Kujawska, M; Madl, T; Kurath-Koller, S; Urlesberger, B; Resch, B; Hall, LJ; Moissl-Eichinger, C Nat Commun. 2023; 14(1):1349 https://doi.org/10.1038/s41467-023-36825-1
Acute appendicitis manifests as two microbiome state types with oral pathogens influencing severity. Blohs, M; Mahnert, A; Brunnader, K; Flucher, C; Castellani, C; Till, H; Singer, G; Moissl-Eichinger, C. Gut Microbes. 2023; 15(1):2145845 https://doi.org/10.1080/19490976.2022.2145845
A catalogue of 1,167 genomes from the human gut archaeome. Chibani, CM; Mahnert, A; Borrel, G; Almeida, A; Werner, A; Brugère, JF; Gribaldo, S; Finn, RD; Schmitz, RA; Moissl-Eichinger, C. Nat Microbiol. 2022; 7(1):48-61 https://doi.org/10.1038/s41564-021-01020-9
Methanogenic archaea in the human gastrointestinal tract. Hoegenauer, C; Hammer, HF; Mahnert, A; Moissl-Eichinger, C. Nat Rev Gastroenterol Hepatol. 2022; 19(12):805-813 https://doi.org/10.1038/s41575-022-00673-z
The sanitary indoor environment-a potential source for intact human-associated anaerobes. Pausan, MR; Blohs, M; Mahnert, A; Moissl-Eichinger, C. NPJ Biofilms Microbiomes. 2022; 8(1):44 https://doi.org/10.1038/s41522-022-00305-z
Archaeal key-residents within the human microbiome: characteristics, interactions and involvement in health and disease. Mohammadzadeh, R; Mahnert, A; Duller, S; Moissl-Eichinger, C. Curr Opin Microbiol. 2022; 67:102146 https://doi.org/10.1016/j.mib.2022.102146
Reduced B12 uptake and increased gastrointestinal formate are associated with archaeome-mediated breath methane emission in humans. Kumpitsch, C; Fischmeister, FPS; Mahnert, A; Lackner, S; Wilding, M; Sturm, C; Springer, A; Madl, T; Holasek, S; Högenauer, C; Berg, IA; Schoepf, V; Moissl-Eichinger, C. Microbiome. 2021; 9(1):193 https://doi.org/10.1186/s40168-021-01130-w